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Anti-PDS5A Antibody
Polyclonal Antibody against HUMAN PDS5A
0.2 mg/ml
Immunohistochemical staining of human gastrointestinal, kidney, placenta and squamous epithelia using Anti-PDS5A antibody HPA036661 (A) shows similar protein distribution across tissues to independent antibody HPA036662 (B).
Product details
- Target protein: PDS5, regulator of cohesion maintenance, homolog A (S. cerevisiae)
- Target gene: PDS5A
- Verified species reactivity: Human
- Interspecies information: Highest antigen sequence indentity to the following orthologs: MOUSE - ENSMUSG00000029202 (90%)RAT - ENSRNOG00000002541 (89%)
- Clonality: Polyclonal
- Isotype: IgG
- Host: Rabbit
- Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
Material Safety Data Sheet - Purification method: Affinity purified using the PrEST antigen as affinity ligand
- Antigen sequence: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
- RSTGTETGSNINVNSELNPSTGNRSREQSSEAAETGVSENEENPVRIISVTPVKNIDPVKNKEINSDQATQGNISSDRGKKRTVTAA
- Notes: Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Current lot
- Unit size: 100µl
- Current lot: R33529
- Concentration: 0.2 mg/ml
Applications
- Immunofluorescence in Cell Lines (ICC-IF) recommended conditions
- Working concentration: 0.25-2 µg/ml
- Fixation/Permeabilization: PFA/Triton X-100
- Immunohistochemistry (IHC) recommended conditions
- Dilution: 1:50 - 1:200
- Retrieval method: HIER pH6
- Western Blot (WB) recommended conditions
- Working concentration: 0.04-0.4 µg/ml
Validation
- Immunofluorescence in Cell Lines (ICC-IF) All ICC-IF characterization data for HPA036661 on the Human Protein Atlas
- Immunohistochemistry (IHC)Validation of protein expression in IHC by comparing independent antibodies targeting different epitopes of the protein. Validated against independent antibody Anti-PDS5A HPA036662 All IHC characterization data in 44 normal tissues and 20 cancers for HPA036661 on the Human Protein Atlas
- Western Blot (WB)Validation of protein expression in IHC by comparing independent antibodies targeting different epitopes of the protein.
Target information
- Protein name: PDS5, regulator of cohesion maintenance, homolog A (S. cerevisiae)
- Gene name: PDS5A
- Alternative gene names: KIAA0648, PIG54, SCC-112
- Ensembl: ENSG00000121892
- Entrez: 23244
- UniProt: Q29RF7
Shipping and storage
- Shipping: Normally shipped at ambient temperatureStorage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
References (1)
- Characterization data on the Human Protein AtlasThis antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.Human Protein Atlas
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Researcher Contributions
Join the Explorer Program Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order. Read more...
Alternative antibodies
Corresponding antigens
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Learn how we validate our antibodies, how we secure their reproducibility, and why we apply enhanced validation. Our antibodies are validated in IHC, ICC-IF, and WB.