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Anti-MDH2 Antibody
Polyclonal Antibody against HUMAN MDH2
0.2 mg/ml
Immunohistochemical staining of human duodenum, fallopian tube, kidney and liver using Anti-MDH2 antibody HPA019716 (A) shows similar protein distribution across tissues to independent antibody HPA019714 (B).
Product details
- Target protein: malate dehydrogenase 2, NAD (mitochondrial)
- Target gene: MDH2
- Verified species reactivity: Human, Mouse, Rat
- Interspecies information: Highest antigen sequence indentity to the following orthologs: MOUSE - ENSMUSG00000019179 (98%)RAT - ENSRNOG00000001440 (98%)
- Clonality: Polyclonal
- Isotype: IgG
- Host: Rabbit
- Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
Material Safety Data Sheet - Purification method: Affinity purified using the PrEST antigen as affinity ligand
- Antigen sequence: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
- LPDCLKGCDVVVIPAGVPRKPGMTRDDLFNTNATIVATLTAACAQHCPEAMICVIANPVNSTIPITAEVFKKHGVYNPNKI
- Notes: Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Current lot
- Unit size: 100µl
- Current lot: C115668
- Concentration: 0.2 mg/ml
Applications
- Immunofluorescence in Cell Lines (ICC-IF) recommended conditions
- Working concentration: 0.25-2 µg/ml
- Fixation/Permeabilization: PFA/Triton X-100
- Immunohistochemistry (IHC) recommended conditions
- Dilution: 1:50 - 1:200
- Retrieval method: HIER pH6
- Western Blot (WB) recommended conditions
- Working concentration: 0.04-0.4 µg/ml
Validation
- Immunofluorescence in Cell Lines (ICC-IF) All ICC-IF characterization data for HPA019716 on the Human Protein Atlas
- Immunohistochemistry (IHC)Validation of protein expression in IHC by comparing independent antibodies targeting different epitopes of the protein. Validated against independent antibodies Anti-MDH2 HPA019714 All IHC characterization data in 44 normal tissues and 20 cancers for HPA019716 on the Human Protein Atlas
- Western Blot (WB)Genetic validation in WB by siRNA knockdown. Validated in Western blot using relevant lysates (see Western blot application images above)
Target information
- Protein name: malate dehydrogenase 2, NAD (mitochondrial)
- Gene name: MDH2
- Ensembl: ENSG00000146701
- Entrez: 4191
- UniProt: P40926
Shipping and storage
- Shipping: Normally shipped at ambient temperatureStorage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
References (4)
- BAP1 Loss Is Associated with Higher ASS1 Expression in Epithelioid Mesothelioma: Implications for Therapeutic StratificationMol Cancer Res , 2023 Jan 20; 21(5):411-427. Epub 2023 Jan 202023 Jan 20
- Characterization of the Role of the Malate Dehydrogenases to Lung Tumor Cell SurvivalJ Cancer , 2017 Jul 5; 8(11):2088-2096. Epub 2017 Jul 52017 Jul 5
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Researcher Contributions
Join the Explorer Program Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order. Read more...
Alternative antibodies
Corresponding antigens
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Learn how we validate our antibodies, how we secure their reproducibility, and why we apply enhanced validation. Our antibodies are validated in IHC, ICC-IF, and WB.