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HPA026638
general transcription factor IIi

Anti-GTF2I Antibody

- ChIP-Certified

Polyclonal Antibody against HUMAN GTF2I

0.1 mg/ml
In Stock (10+)
Delivery time: Ready to Ship
4 311,0 kr
Lowest price during the previous 30 days before discount: 4 106,0 kr
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Immunohistochemical staining of human stomach shows strong nuclear positivity in glandular cells.

Immunohistochemical staining of human stomach shows strong nuclear positivity in glandular cells.

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Product details

  • Target protein: general transcription factor IIi
  • Target gene: GTF2I
  • Verified species reactivity: Human
  • Interspecies information: Highest antigen sequence indentity to the following orthologs: MOUSE - ENSMUSG00000060261 (100%)RAT - ENSRNOG00000001479 (99%)
  • Clonality: Polyclonal
  • Isotype: IgG
  • Host: Rabbit
  • Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
    Material Safety Data Sheet
  • Purification method: Affinity purified using the PrEST antigen as affinity ligand
  • Antigen sequence: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • FVIKKHELLNSTREDLQLDKPASGVKEEWYARITKLRKMVDQLFCKKFAEALGSTEAKAVPYQKFEAHPNDLYVEGLPENIPFRSPSWYGIPRLEKIIQVGNRIKFVIKRPELLTHSTTEVTQPRTNTPVKEDWNVRIT
  • Notes: Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Current lot

  • Unit size: 100µl
  • Current lot: A106193
  • Concentration: 0.1 mg/ml

Applications

  • Chromatin Immunoprecipitation (ChIP) recommended conditions
    • Working concentration:  1-10µg per reaction
  • Immunofluorescence in Cell Lines (ICC-IF) recommended conditions
    • Working concentration: 0.25-2 µg/ml
    • Fixation/Permeabilization: PFA/Triton X-100
  • Immunohistochemistry (IHC) recommended conditions
    • Dilution: 1:20 - 1:50
    • Retrieval method: HIER pH6
  • Western Blot (WB) recommended conditions
    • Working concentration: 0.04-0.4 µg/ml

Validation

Target information

  • Protein name: general transcription factor IIi
  • Gene name: GTF2I
  • Alternative gene names: BAP-135BTKAP1DIWSIB291SPINTFII-IWBSCR6
  • Ensembl: ENSG00000263001
  • Entrez: 2969
  • UniProt: P78347

Shipping and storage

  • Shipping: Normally shipped at ambient temperatureStorage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References (2)

  • Identification of proximal SUMO-dependent interactors using SUMO-IDBarroso-Gomila O, Trulsson F, Muratore V, Canosa I, Merino-Cacho L, Cortazar AR, Pérez C, Azkargorta M, Iloro I, Carracedo A, Aransay AM, Elortza F, Mayor U, Vertegaal AC, Barrio R, Sutherland JDNat Commun , 2021 Nov 18; 12:6671. Epub 2021 Nov 182021 Nov 18
  • Characterization data on the Human Protein AtlasThis antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.Human Protein Atlas

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Researcher Contributions

    Join the Explorer Program Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order. Read more...

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