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Anti-GPRC5B Antibody
Polyclonal Antibody against HUMAN GPRC5B
0.2 mg/ml
Immunohistochemistry analysis in human cerebral cortex and liver tissues using HPA015247 antibody. Corresponding GPRC5B RNA-seq data are presented for the same tissues.
Product details
- Target protein: G protein-coupled receptor, class C, group 5, member B
- Target gene: GPRC5B
- Verified species reactivity: Human
- Interspecies information: Highest antigen sequence indentity to the following orthologs: MOUSE - ENSMUSG00000008734 (82%)RAT - ENSRNOG00000016013 (83%)
- Clonality: Polyclonal
- Isotype: IgG
- Host: Rabbit
- Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
Material Safety Data Sheet - Purification method: Affinity purified using the PrEST antigen as affinity ligand
- Antigen sequence: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
- IHCTLLPALQENTPNYFDTSQPRMRETAFEEDVQLPRAYMENKAFSMDEHNAALRTAGFPNGSLGKRPSGSLGKRPSAPFRSNVYQPTEMAVVLNGGTIPTAP
- Notes: Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Current lot
- Unit size: 100µl
- Current lot: 000004503
- Concentration: 0.2 mg/ml
Applications
- Immunofluorescence in Cell Lines (ICC-IF) recommended conditions
- Working concentration: 0.25-2 µg/ml
- Fixation/Permeabilization: PFA/Triton X-100
- Immunohistochemistry (IHC) recommended conditions
- Dilution: 1:1000 - 1:2500
- Retrieval method: HIER pH6
Validation
- Immunofluorescence in Cell Lines (ICC-IF) All ICC-IF characterization data for HPA015247 on the Human Protein Atlas
- Immunohistochemistry (IHC)Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues. All IHC characterization data in 44 normal tissues and 20 cancers for HPA015247 on the Human Protein Atlas
Target information
- Protein name: G protein-coupled receptor, class C, group 5, member B
- Gene name: GPRC5B
- Alternative gene names: RAIG-2
- Ensembl: ENSG00000167191
- Entrez: 51704
- UniProt: Q9NZH0
Shipping and storage
- Shipping: Normally shipped at ambient temperatureStorage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
References (3)
- GPRC5B protects osteoarthritis by regulation of autophagy signalingActa Pharm Sin B None2023 May 14
- GPRC5b Modulates Inflammatory Response in Glomerular Diseases via NF-κB PathwayJ Am Soc Nephrol , 2019 Jul 8; 30(9):1573-1586. Epub 2019 Jul 82019 Jul 8
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Researcher Contributions
Join the Explorer Program Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order. Read more...
Corresponding antigens
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Learn how we validate our antibodies, how we secure their reproducibility, and why we apply enhanced validation. Our antibodies are validated in IHC, ICC-IF, and WB.