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HPA034765
G elongation factor, mitochondrial 1

Anti-GFM1 Antibody

Polyclonal Antibody against HUMAN GFM1

0.1 mg/ml
In Stock (10+)
Delivery time: Ready to Ship
USD 562.0
Lowest price during the previous 30 days before discount: USD 535.0
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Immunohistochemical staining of human kidney, placenta, stomach and testis using Anti-GFM1 antibody HPA034765 (A) shows similar protein distribution across tissues to independent antibody HPA034764 (B).

Immunohistochemical staining of human kidney, placenta, stomach and testis using Anti-GFM1 antibody HPA034765 (A) shows similar protein distribution across tissues to independent antibody HPA034764 (B).

Product details

  • Target protein: G elongation factor, mitochondrial 1
  • Target gene: GFM1
  • Verified species reactivity: Human
  • Interspecies information: Highest antigen sequence indentity to the following orthologs: MOUSE - ENSMUSG00000027774 (96%)RAT - ENSRNOG00000012873 (96%)
  • Clonality: Polyclonal
  • Isotype: IgG
  • Host: Rabbit
  • Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
    Material Safety Data Sheet
  • Purification method: Affinity purified using the PrEST antigen as affinity ligand
  • Antigen sequence: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • QYGKVIGVLEPLDPEDYTKLEFSDETFGSNIPKQFVPAVEKGFLDACEKGPLSGHKLSGLRFVLQDGAHHMVDSNEISFIRAGE
  • Notes: Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Current lot

  • Unit size: 100µl
  • Current lot: 000014502
  • Concentration: 0.1 mg/ml

Applications

  • Immunohistochemistry (IHC) recommended conditions
    • Dilution: 1:20 - 1:50
    • Retrieval method: HIER pH6
  • Western Blot (WB) recommended conditions
    • Working concentration: 0.04-0.4 µg/ml

Validation

Target information

  • Protein name: G elongation factor, mitochondrial 1
  • Gene name: GFM1
  • Alternative gene names: EFGMEGF1GFM
  • Ensembl: ENSG00000168827
  • Entrez: 85476
  • UniProt: Q96RP9

Shipping and storage

  • Shipping: Normally shipped at ambient temperatureStorage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References (2)

  • Identification of Elongation Factor G as the Conserved Cellular Target of Argyrin BNyfeler B, Hoepfner D, Palestrant D, Kirby CA, Whitehead L, Yu R, Deng G, Caughlan RE, Woods AL, Jones AK, Barnes SW, Walker JR, Gaulis S, Hauy E, Brachmann SM, Krastel P, Studer C, Riedl R, Estoppey D, Aust T, Movva NR, Wang Z, Salcius M, Michaud GA, McAllister G, Murphy LO, Tallarico JA, Wilson CJ, Dean CRPLoS One , 2012; 7(9):e42657. Epub 2012 Sep 102012
  • Characterization data on the Human Protein AtlasThis antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.Human Protein Atlas

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Researcher Contributions

    Join the Explorer Program Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order. Read more...

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