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HPA041478
enkurin domain containing 1

Anti-ENKD1 Antibody

Polyclonal Antibody against HUMAN ENKD1

1.4 mg/ml
In Stock (10+)
Delivery time: Ready to Ship
4 570,0 kr
Lowest price during the previous 30 days before discount: 4 352,0 kr
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Immunohistochemical staining of human fallopian tube shows moderate cytoplasmic positivity in glandular cells.

Immunohistochemical staining of human fallopian tube shows moderate cytoplasmic positivity in glandular cells.

Product details

  • Target protein: enkurin domain containing 1
  • Target gene: ENKD1
  • Verified species reactivity: Human
  • Interspecies information: Highest antigen sequence indentity to the following orthologs: MOUSE - ENSMUSG00000013155 (87%)RAT - ENSRNOG00000024364 (92%)
  • Clonality: Polyclonal
  • Isotype: IgG
  • Host: Rabbit
  • Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
    Material Safety Data Sheet
  • Purification method: Affinity purified using the PrEST antigen as affinity ligand
  • Antigen sequence: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • QVLAQVLEQQRQAQEHYNATQKGHVPHYLLERRDLWRREAEARKQSQPDPAMPPGHTRMPENQRLETLTKL
  • Notes: Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Current lot

  • Unit size: 100µl
  • Current lot: 000021102
  • Concentration: 1.4 mg/ml

Applications

  • Immunofluorescence in Cell Lines (ICC-IF) recommended conditions
    • Working concentration: 0.25-2 µg/ml
    • Fixation/Permeabilization: PFA/Triton X-100
  • Immunohistochemistry (IHC) recommended conditions
    • Dilution: 1:200 - 1:500
    • Retrieval method: HIER pH6
  • Western Blot (WB) recommended conditions
    • Working concentration: 0.04-0.4 µg/ml

Validation

Target information

  • Protein name: enkurin domain containing 1
  • Gene name: ENKD1
  • Alternative gene names: C16orf48DKFZP434A1319
  • Ensembl: ENSG00000124074
  • Entrez: 84080
  • UniProt: Q9H0I2

Shipping and storage

  • Shipping: Normally shipped at ambient temperatureStorage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References (3)

  • ENKD1 promotes CP110 removal through competing with CEP97 to initiate ciliogenesisSong T, Yang Y, Zhou P, Ran J, Zhang L, Wu X, Xie W, Zhong T, Liu H, Liu M, Li D, Zhao H, Zhou JEMBO Rep , 2022 Mar 18; 23(5):e54090. Epub 2022 Mar 182022 Mar 18
  • Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells.Stadler C, Rexhepaj E, Singan VR, Murphy RF, Pepperkok R, Uhlén M, Simpson JC, Lundberg ENat Methods , 2013 Apr; 10(4):315-23. Epub 2013 Feb 242013 Apr
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Researcher Contributions

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