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Anti-EIF6 Antibody
Polyclonal Antibody against HUMAN EIF6
0.1 mg/ml
Immunohistochemistry analysis in human skin and skeletal muscle tissues using HPA040873 antibody. Corresponding EIF6 RNA-seq data are presented for the same tissues.
Product details
- Target protein: eukaryotic translation initiation factor 6
- Target gene: EIF6
- Verified species reactivity: Human, Mouse, Rat
- Interspecies information: Highest antigen sequence indentity to the following orthologs: MOUSE - ENSMUSG00000027613 (100%)RAT - ENSRNOG00000049497 (100%)
- Clonality: Polyclonal
- Isotype: IgG
- Host: Rabbit
- Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
Material Safety Data Sheet - Purification method: Affinity purified using the PrEST antigen as affinity ligand
- Antigen sequence: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
- LSALGNVTTCNDYVALVHPDLDRETEEILADVLKVEVFRQTVADQVLVGSYCVFSNQGGLVHPKTSIEDQDELSSLLQVP
- Notes: Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Current lot
- Unit size: 100µl
- Current lot: 000020841
- Concentration: 0.1 mg/ml
Applications
- Immunofluorescence in Cell Lines (ICC-IF) recommended conditions
- Working concentration: 0.25-2 µg/ml
- Fixation/Permeabilization: PFA/Triton X-100
- Immunohistochemistry (IHC) recommended conditions
- Dilution: 1:1000 - 1:2500
- Retrieval method: HIER pH6
- Western Blot (WB) recommended conditions
- Working concentration: 0.04-0.4 µg/ml
Validation
- Immunofluorescence in Cell Lines (ICC-IF) All ICC-IF characterization data for HPA040873 on the Human Protein Atlas
- Immunohistochemistry (IHC)Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues. All IHC characterization data in 44 normal tissues and 20 cancers for HPA040873 on the Human Protein Atlas
- Western Blot (WB)Validated in Western blot using relevant lysates (see Western blot application images above)
Target information
- Protein name: eukaryotic translation initiation factor 6
- Gene name: EIF6
- Alternative gene names: b(2)gcn, EIF3A, ITGB4BP, p27BBP
- Ensembl: ENSG00000242372
- Entrez: 3692
- UniProt: P56537
Shipping and storage
- Shipping: Normally shipped at ambient temperatureStorage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
References (2)
- eIF6 promotes the malignant progression of human hepatocellular carcinoma via the mTOR signaling pathwayJ Transl Med , 2021 May 20; 19:216. Epub 2021 May 202021 May 20
- Characterization data on the Human Protein AtlasThis antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.Human Protein Atlas
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Researcher Contributions
Join the Explorer Program Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order. Read more...
Corresponding antigens
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Learn how we validate our antibodies, how we secure their reproducibility, and why we apply enhanced validation. Our antibodies are validated in IHC, ICC-IF, and WB.