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HPA002637
cyclin-dependent kinase 6

Anti-CDK6 Antibody

Polyclonal Antibody against HUMAN CDK6

0.1 mg/ml
In Stock (10+)
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USD 530.0
Lowest price during the previous 30 days before discount: USD 505.0
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Immunohistochemistry analysis in human placenta and skeletal muscle tissues using HPA002637 antibody. Corresponding CDK6 RNA-seq data are presented for the same tissues.

Immunohistochemistry analysis in human placenta and skeletal muscle tissues using HPA002637 antibody. Corresponding CDK6 RNA-seq data are presented for the same tissues.

Product details

  • Target protein: cyclin-dependent kinase 6
  • Target gene: CDK6
  • Verified species reactivity: Human
  • Interspecies information: Highest antigen sequence indentity to the following orthologs: MOUSE - ENSMUSG00000040274 (92%)RAT - ENSRNOG00000009258 (92%)
  • Clonality: Polyclonal
  • Isotype: IgG
  • Host: Rabbit
  • Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
    Material Safety Data Sheet
  • Purification method: Affinity purified using the PrEST antigen as affinity ligand
  • Antigen sequence: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • FRGSSDVDQLGKILDVIGLPGEEDWPRDVALPRQAFHSKSAQPIEKFVTDIDELGKDLLLKCLTFNPAKRISAYSALSHPYFQDLERCKENLDSHLPPSQNTSELN
  • Notes: Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Current lot

  • Unit size: 100µl
  • Current lot: 000052257
  • Concentration: 0.1 mg/ml

Applications

  • Immunofluorescence in Cell Lines (ICC-IF) recommended conditions
    • Working concentration: 0.25-2 µg/ml
    • Fixation/Permeabilization: PFA/Triton X-100
  • Immunohistochemistry (IHC) recommended conditions
    • Dilution: 1:50 - 1:200
    • Retrieval method: HIER pH6
  • Western Blot (WB) recommended conditions
    • Working concentration: 0.04-0.4 µg/ml

Validation

Target information

  • Protein name: cyclin-dependent kinase 6
  • Gene name: CDK6
  • Alternative gene names: PLSTIRE
  • Ensembl: ENSG00000105810
  • Entrez: 1021
  • UniProt: Q00534

Shipping and storage

  • Shipping: Normally shipped at ambient temperatureStorage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References (6)

  • INK4 tumor suppressor proteins mediate resistance to CDK4/6 kinase inhibitorsLi Q, Jiang B, Guo J, Shao H, Priore IS, Chang Q, Kudo R, Li Z, Razavi P, Liu B, Boghossian AS, Rees MG, Ronan MM, Roth JA, Donovan KA, Palafox M, Reis-Filho JS, de Stanchina E, Fischer ES, Rosen N, Serra V, Koff A, Chodera JD, Gray NS, Chandarlapaty SCancer Discov , 2021 Sep 20; 12(2):356-371. Epub 2021 Sep 202021 Sep 20
  • Requirement of DNMT1 to orchestrate epigenomic reprogramming for NPM-ALK–driven lymphomagenesisRedl E, Sheibani-Tezerji R, Cardona CD, Hamminger P, Timelthaler G, Hassler MR, Zrimšek M, Lagger S, Dillinger T, Hofbauer L, Draganić K, Tiefenbacher A, Kothmayer M, Dietz CH, Ramsahoye BH, Kenner L, Bock C, Seiser C, Ellmeier W, Schweikert G, Egger GLife Sci Alliance , 2020 Dec 11; 4(2):e202000794. Epub 2020 Dec 112020 Dec 11
Did we miss your publication? Have you published using HPA002637? Please let us know and we will be happy to include your reference on this page. Submit reference

Researcher Contributions

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