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Anti-NOP56 Antibody
Monoclonal Antibody against HUMAN NOP56
1.0 mg/ml
Immunohistochemical staining of human prostate cancer shows strong nucleolar positivity in glandular cells.
Product details
- Target protein: NOP56 ribonucleoprotein homolog (yeast)
- Target gene: NOP56
- Verified species reactivity: Human
- Interspecies information: Highest antigen sequence indentity to the following orthologs: MOUSE - ENSMUSG00000027405 (59%)RAT - ENSRNOG00000007128 (62%)
- Clonality: Monoclonal
- Clone ID: CL2603
- Isotype: IgG1
- Host: Mouse
- Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
Material Safety Data Sheet - Purification method: Protein A purified
- Notes: Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Current lot
- Unit size: 100µl
- Current lot: MAB-03517
- Concentration: 1.0 mg/ml
Applications
- Immunofluorescence in Cell Lines (ICC-IF) recommended conditions
- Working concentration: 2-10 µg/ml
- Fixation/Permeabilization: PFA/Triton X-100
- Immunohistochemistry (IHC) recommended conditions
- Dilution: 1:500 - 1:1000
- Retrieval method: HIER pH6
- Western Blot (WB) recommended conditions
- Working concentration: 1 µg/ml
Validation
- Immunofluorescence in Cell Lines (ICC-IF) All ICC-IF characterization data for AMAb91013 on the Human Protein Atlas
- Immunohistochemistry (IHC) All IHC characterization data in 44 normal tissues and 20 cancers for AMAb91013 on the Human Protein Atlas
- Western Blot (WB)Genetic validation in WB by siRNA knockdown. Validated in Western blot using relevant lysates (see Western blot application images above)
Target information
- Protein name: NOP56 ribonucleoprotein homolog (yeast)
- Gene name: NOP56
- Alternative gene names: NOL5A, SCA36
- Ensembl: ENSG00000101361
- Entrez: 100302138
- UniProt: O00567
Shipping and storage
- Shipping: Normally shipped at ambient temperatureStorage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
References (3)
- Prenylcysteine oxidase 1, an emerging player in atherosclerosisCommun Biol , 2021 Sep 21; 4:1109. Epub 2021 Sep 212021 Sep 21
- Expression, Localization, and Function of the Nucleolar Protein BOP1 in Prostate Cancer ProgressionAm J Pathol , 2021 Jan; 191(1):168-1792021 Jan
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Researcher Contributions
Join the Explorer Program Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order. Read more...
Alternative antibodies
Corresponding antigens
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Learn how we validate our antibodies, how we secure their reproducibility, and why we apply enhanced validation. Our antibodies are validated in IHC, ICC-IF, and WB.