VRK1 Antibody (HPA000660)

VRK1 Antibody (HPA000660)


vaccinia related kinase 1
100 µl
Available in stock
Alternative names
Species reactivity
Alternative Antibodies
  • Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferus ducts.
Recommended conditions:
  • Dilution: 1:200 - 1:500
  • Retrieval method: HIER pH6
Additional IHC Characterization Data
Access additional IHC-data for Anti-VRK1 on the Human Protein Atlas. View manually annotated IHC-data from 576 human tissue samples of normal and cancer origin as well as duplicate samples from 59 clinical cell samples and cell lines.
  • Lane 1: Marker [kDa] 207, 110, 79, 49, 32, 25, 17
    Lane 2: Human cell line RT-4
    Lane 3: Human cell line EFO-21
Western Blot
Recommended conditions:
  • Dilution: 1:250 - 1:500
Additional WB Characterization Data
Access the Western Blot validation data for Anti-VRK1 on the Human Protein Atlas. Use the Antigen View to display the antigen location on the target protein for all splice variants.
  • Immunofluorescent staining of human cell line U-251MG shows positivity in nucleus but not nucleoli.
Recommended conditions:
  • Fixation/Permeabilization: PFA/Triton X-100
  • Working concentration: 1-4 µg/ml
Additional IF Characterization Data
Access additional IF characterization data for Anti-VRK1 on the Human Protein Atlas. View manually annotated subcellular localization data in three cell lines selected based on RNA sequencing data.
Antigen Sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence:
Purification Method
Affinity purified using the PrEST antigen as affinity ligand
Specific reactivity against target PrEST antigen validated on protein array with 384 randomly selected antigens.
Interspecies Information Highest antigen sequence identity to the following orthologos:
The antibodies are delivered in 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Vial Size 100 µl
Current Lot
0.1 mg/ml
Storage Store at +4°C for short term storage (1-2 days). Long time storage is recommended at -20°C. If stored at lower temperature, aliquot to avoid repeated freezing and thawing. Working dilution samples should be discarded if not used within 12 hours.
Normally shipped at ambient temperature
The antibody solution should be gently mixed before use. Optimal concentrations and conditions for each application should be determined by the user.

Human Protein Atlas

This antibody has been used for staining of 48 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

Kim IJ, Quigley D, To MD, Pham P, Lin K, Jo B, Jen KY, Raz D, Kim J, Mao JH, Jablons D, Balmain A Rewiring of human lung cell lineage and mitotic networks in lung adenocarcinomas. Nat Commun 2013;4:1701 PubMed ID: 23591868 DOI: 10.1038/ncomms2660
Sanz-García M, Monsalve DM, Sevilla A, Lazo PA Vaccinia-related Kinase 1 (VRK1) Is an Upstream Nucleosomal Kinase Required for the Assembly of 53BP1 Foci in Response to Ionizing Radiation-induced DNA Damage J Biol Chem 2012 Jul 6; 287(28):23757-23768. Epub 2012/05/22. PubMed ID: 22621922 DOI: 10.1074/jbc.M112.353102
T P Molitor, P Traktman Molecular genetic analysis of VRK1 in mammary epithelial cells: depletion slows proliferation <i>in vitro</i> and tumor growth and metastasis <i>in vivo</i> Oncogenesis 2013-06-03 2;(6):e48- PubMed ID: 0 DOI: 10.1038/oncsis.2013.11

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100 researchers are working within the Human Protein Atlas project. Our Scientific Support Team provides you with answers or guide you to the right people

If you have questions or comments regarding this antibody please contact our Scientific Support: contact@atlasantibodies.com.

Are you using our antibodies in an application or in a species we have not yet validated? Go to our Technical Support and Resources page and read about our Explorer Program.


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