ME2 Antibody (HPA008880)

ME2 Antibody (HPA008880)


malic enzyme 2, NAD(+)-dependent, mitochondrial
100 µl
Available in stock
Alternative names
Species reactivity
Alternative Antibodies
  • Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in glandular cells.
Recommended conditions:
  • Dilution: 1:20 - 1:50
  • Retrieval method: HIER pH6
Additional IHC Characterization Data
Access additional IHC-data for Anti-ME2 on the Human Protein Atlas. View manually annotated IHC-data from 576 human tissue samples of normal and cancer origin as well as duplicate samples from 59 clinical cell samples and cell lines.
  • Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
    Lane 2: Human cell line RT-4
    Lane 3: Human cell line U-251MG sp
Western Blot
Recommended conditions:
  • Dilution: 1:100 - 1:250
Additional WB Characterization Data
Access the Western Blot validation data for Anti-ME2 on the Human Protein Atlas. Use the Antigen View to display the antigen location on the target protein for all splice variants.
  • Immunofluorescent staining of human cell line U-2 OS shows positivity in mitochondria & vesicles.
Recommended conditions:
  • Fixation/Permeabilization: PFA/Triton X-100
  • Working concentration: 1-4 µg/ml
Additional IF Characterization Data
Access additional IF characterization data for Anti-ME2 on the Human Protein Atlas. View manually annotated subcellular localization data in three cell lines selected based on RNA sequencing data.
Antigen Sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence:
Purification Method
Affinity purified using the PrEST antigen as affinity ligand
Specific reactivity against target PrEST antigen validated on protein array with 384 randomly selected antigens.
Interspecies Information Highest antigen sequence identity to the following orthologos:
The antibodies are delivered in 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Vial Size 100 µl
Current Lot
0.1 mg/ml
Storage Store at +4°C for short term storage (1-2 days). Long time storage is recommended at -20°C. If stored at lower temperature, aliquot to avoid repeated freezing and thawing. Working dilution samples should be discarded if not used within 12 hours.
Normally shipped at ambient temperature
The antibody solution should be gently mixed before use. Optimal concentrations and conditions for each application should be determined by the user.

Human Protein Atlas

This antibody has been used for staining of 48 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

Zoccarato F, Cavallini L, Alexandre A Succinate is the controller of O2-/H2O2 release at mitochondrial complex I : negative modulation by malate, positive by cyanide. J Bioenerg Biomembr 2009 Aug;41(4):387-93 PubMed ID: 19821037 DOI: 10.1007/s10863-009-9238-2
Brown LJ, Longacre MJ, Hasan NM, Kendrick MA, Stoker SW, Macdonald MJ Chronic reduction of the cytosolic or mitochondrial NAD(P)-malic enzyme does not affect insulin secretion in a rat insulinoma cell line. J Biol Chem 2009 Dec 18;284(51):35359-67 PubMed ID: 19858194 DOI: 10.1074/jbc.M109.040394
MacDonald MJ, Longacre MJ, Kendrick MA Mitochondrial Malic Enzyme (ME2) In Pancreatic Islets of the Human, Rat and Mouse and Clonal Insulinoma Cells: Simple Enzyme Assay For Mitochondrial Malic Enzyme 2 Arch Biochem Biophys 2009 Aug 15; 488(2):100-104. PubMed ID: 19691144 DOI: 10.1016/
MacDonald MJ, Longacre MJ, Kendrick MA Mitochondrial malic enzyme (ME2) in pancreatic islets of the human, rat and mouse and clonal insulinoma cells. Arch Biochem Biophys 2009 Aug 15;488(2):100-4 PubMed ID: 19691144

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