IL18 Antibody (HPA003980)

IL18 Antibody (HPA003980)

Anti-IL18

interleukin 18 (interferon-gamma-inducing factor)
Anti-IL18
HPA003980
100 µl
$429.00
Available in stock
Clonality
Polyclonal
Alternative names
IGIF, IL-18, IL-1g, IL1F4
Species reactivity
Human
Close
  • Immunohistochemical staining of human lymph node shows secreted positivity.
Immunohistochemistry
Recommended conditions:
  • Dilution: 1:200 - 1:500
  • Retrieval method: HIER pH6
Additional IHC Characterization Data
Access additional IHC-data for Anti-IL18 on the Human Protein Atlas. View manually annotated IHC-data from 576 human tissue samples of normal and cancer origin as well as duplicate samples from 59 clinical cell samples and cell lines.
Close
  • Lane 1: Marker [kDa] 230, 110, 82, 49, 32, 26, 18
    Lane 2: Human cell line RT-4
    Lane 3: Human cell line U-251MG sp
Western Blot
Recommended conditions:
  • Dilution: 1:250 - 1:500
Additional WB Characterization Data
Access the Western Blot validation data for Anti-IL18 on the Human Protein Atlas. Use the Antigen View to display the antigen location on the target protein for all splice variants.
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Antigen Sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence:
MAAEPVEDNCINFVAMKFIDNTLYFIAEDDENLESDYFGKLESKLSVIRNLNDQVLFIDQGNRPLFEDMTDSDCRDNAPRTIFIISMYKDSQPRGMAVTISVKCEKISTLSCENKIISFKEMNPPDNIKDTKSDIIFFQRSVPGHDNKMQF
Purification Method
Affinity purified using the PrEST antigen as affinity ligand
Specificity
Specific reactivity against target PrEST antigen validated on protein array with 384 randomly selected antigens.
Interspecies Information Highest antigen sequence identity to the following orthologos:
Form
The antibodies are delivered in 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Vial Size 100 µl
Current Lot
A78490
Concentration
0.2 mg/ml
Storage Store at +4°C for short term storage (1-2 days). Long time storage is recommended at -20°C. If stored at lower temperature, aliquot to avoid repeated freezing and thawing. Working dilution samples should be discarded if not used within 12 hours.
Shipping
Normally shipped at ambient temperature
Notes
The antibody solution should be gently mixed before use. Optimal concentrations and conditions for each application should be determined by the user.

Human Protein Atlas

This antibody has been used for staining of 48 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

Kato BS, Nicholson G, Neiman M, Rantalainen M, Holmes CC, Barrett A, Uhlén M, Nilsson P, Spector TD, Schwenk JM Variance decomposition of protein profiles from antibody arrays using a longitudinal twin model. Proteome Sci 2011 Nov 17;9:73 PubMed ID: 22093360 DOI: 10.1186/1477-5956-9-73
Estes JD, Harris LD, Klatt NR, Tabb B, Pittaluga S, Paiardini M, Barclay GR, Smedley J, Pung R, Oliveira KM, Hirsch VM, Silvestri G, Douek DC, Miller CJ, Haase AT, Lifson J, Brenchley JM Damaged intestinal epithelial integrity linked to microbial translocation in pathogenic simian immunodeficiency virus infections. PLoS Pathog 2010 Aug 19;6(8):e1001052 PubMed ID: 20808901 DOI: 10.1371/journal.ppat.1001052

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If you have questions or comments regarding this antibody please contact our Scientific Support: contact@atlasantibodies.com.

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