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Anti-SPEF2 Antibody
Polyclonal Antibody against HUMAN SPEF2
0.1 mg/ml
Immunohistochemistry analysis in human fallopian tube and skeletal muscle tissues using HPA039606 antibody. Corresponding SPEF2 RNA-seq data are presented for the same tissues.
Product details
- Target protein: sperm flagellar 2
- Target gene: SPEF2
- Verified species reactivity: Human
- Interspecies information: Highest antigen sequence indentity to the following orthologs: MOUSE - ENSMUSG00000072663 (82%)RAT - ENSRNOG00000058275 (78%)
- Clonality: Polyclonal
- Isotype: IgG
- Host: Rabbit
- Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
Material Safety Data Sheet - Purification method: Affinity purified using the PrEST antigen as affinity ligand
- Antigen sequence: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
- VPQPPKPGSEEWVYVNEPVPEEMPLFLVPYWELIENSYINTIKTVLRHLREDQHTVLAYLYEIRTSFQEFLKRPDHKQDFVAQWQ
- Notes: Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Current lot
- Unit size: 100µl
- Current lot: 000057998
- Concentration: 0.1 mg/ml
Applications
- Immunohistochemistry (IHC) recommended conditions
- Dilution: 1:1000 - 1:2500
- Retrieval method: HIER pH6
Validation
- Immunohistochemistry (IHC)Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues. Validation of protein expression in IHC by comparing independent antibodies targeting different epitopes of the protein. Validated against independent antibody Anti-SPEF2 HPA040343 All IHC characterization data in 44 normal tissues and 20 cancers for HPA039606 on the Human Protein Atlas
Target information
- Protein name: sperm flagellar 2
- Gene name: SPEF2
- Alternative gene names: CT122, FLJ23577, KPL2
- Ensembl: ENSG00000152582
- Entrez: 79925
- UniProt: Q9C093
Shipping and storage
- Shipping: Normally shipped at ambient temperatureStorage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
References (4)
- HYDIN variants cause primary ciliary dyskinesia in the Finnish populationPediatr Pulmonol , 2024 Sep 18; 59(12):3601-3609. Epub 2024 Sep 182024 Sep 18
- Novel SPEF2 Variant in a Japanese Patient with Primary Ciliary Dyskinesia: A Case Report and Literature ReviewJ Clin Med , 2022 Dec 31; 12(1):317. Epub 2022 Dec 312022 Dec 31
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Researcher Contributions
Join the Explorer Program Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order. Read more...
Alternative antibodies
Corresponding antigens
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Learn how we validate our antibodies, how we secure their reproducibility, and why we apply enhanced validation. Our antibodies are validated in IHC, ICC-IF, and WB.