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HPA029273
suppressor of Ty 5 homolog (S. cerevisiae)

Anti-SUPT5H Antibody

- ChIP-Certified

Polyclonal Antibody against HUMAN SUPT5H

0.1 mg/ml
In Stock (10+)
Delivery time: Ready to Ship
4 570,0 kr
Lowest price during the previous 30 days before discount: 4 352,0 kr
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Immunohistochemistry analysis in human testis and pancreas tissues using Anti-SUPT5H antibody. Corresponding SUPT5H RNA-seq data are presented for the same tissues.

Immunohistochemistry analysis in human testis and pancreas tissues using Anti-SUPT5H antibody. Corresponding SUPT5H RNA-seq data are presented for the same tissues.

Product details

  • Target protein: suppressor of Ty 5 homolog (S. cerevisiae)
  • Target gene: SUPT5H
  • Verified species reactivity: Human, Mouse, Rat
  • Interspecies information: Highest antigen sequence indentity to the following orthologs: MOUSE - ENSMUSG00000003435 (96%)RAT - ENSRNOG00000032034 (96%)
  • Clonality: Polyclonal
  • Isotype: IgG
  • Host: Rabbit
  • Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
    Material Safety Data Sheet
  • Purification method: Affinity purified using the PrEST antigen as affinity ligand
  • Antigen sequence: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • VGYSPMTPGAPSPGGYNPHTPGSGIEQNSSDWVTTDIQVKVRDTYLDTQVVGQTGVIRSVTGGMCSVYLKDSEKVVSISSEHLEPITPTKNNKVKVILGEDREATGVLLSIDGEDGIVRMDLDEQLKILNLRFL
  • Notes: Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Current lot

  • Unit size: 100µl
  • Current lot: 000012280
  • Concentration: 0.1 mg/ml

Applications

  • Chromatin Immunoprecipitation (ChIP) recommended conditions
    • Working concentration:  1-10µg per reaction
  • Immunofluorescence in Cell Lines (ICC-IF) recommended conditions
    • Working concentration: 0.25-2 µg/ml
    • Fixation/Permeabilization: PFA/Triton X-100
  • Immunohistochemistry (IHC) recommended conditions
    • Dilution: 1:50 - 1:200
    • Retrieval method: HIER pH6
  • Western Blot (WB) recommended conditions
    • Working concentration: 0.04-0.4 µg/ml

Validation

Target information

  • Protein name: suppressor of Ty 5 homolog (S. cerevisiae)
  • Gene name: SUPT5H
  • Alternative gene names: FLJ34157SPT5SPT5H
  • Ensembl: ENSG00000196235
  • Entrez: 6829
  • UniProt: O00267

Shipping and storage

  • Shipping: Normally shipped at ambient temperatureStorage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References (3)

  • Proteomic screen reveals Fbw7 as a modulator of the NF-κB pathway.Arabi A, Ullah K, Branca RM, Johansson J, Bandarra D, Haneklaus M, Fu J, Ariës I, Nilsson P, Den Boer ML, Pokrovskaja K, Grandér D, Xiao G, Rocha S, Lehtiö J, Sangfelt ONat Commun , 2012; 3:9762012
  • Suppressor of Ty homolog-5, a novel tumor-specific human telomerase reverse transcriptase promoter-binding protein and activator in colon cancer cellsChen R, Zhu J, Dong Y, He C, Hu XOncotarget , 1/01/01; 6(32):32841-32855. Epub 2015 Sep 251/01/01
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Researcher Contributions

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