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Anti-PPP1R8 Antibody
Polyclonal Antibody against HUMAN PPP1R8
0.1 mg/ml
Western blot analysis in human cell line NTERA-2.
Product details
- Target protein: protein phosphatase 1, regulatory subunit 8
- Target gene: PPP1R8
- Verified species reactivity: Human
- Interspecies information: Highest antigen sequence indentity to the following orthologs: MOUSE - ENSMUSG00000028882 (100%)RAT - ENSRNOG00000059550 (100%)
- Clonality: Polyclonal
- Isotype: IgG
- Host: Rabbit
- Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
Material Safety Data Sheet - Purification method: Affinity purified using the PrEST antigen as affinity ligand
- Antigen sequence: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
- TFLGHIRLEPHKPQQIPIDSTVSFGASTRAYTLREKPQTLPSAVKGDEKMGGEDDELKGLLGLPEEETELDNLTEFNTAHNK
- Notes: Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Current lot
- Unit size: 100µl
- Current lot: 000053546
- Concentration: 0.1 mg/ml
Applications
- Immunofluorescence in Cell Lines (ICC-IF) recommended conditions
- Working concentration: 0.25-2 µg/ml
- Fixation/Permeabilization: PFA/Triton X-100
- Western Blot (WB) recommended conditions
- Working concentration: 0.04-0.4 µg/ml
Validation
- Immunofluorescence in Cell Lines (ICC-IF) All ICC-IF characterization data for HPA027452 on the Human Protein Atlas
- Western Blot (WB)Validated in Western blot using relevant lysates (see Western blot application images above)
Target information
- Protein name: protein phosphatase 1, regulatory subunit 8
- Gene name: PPP1R8
- Alternative gene names: ard-1, ARD1, NIPP-1, NIPP1, PRO2047
- Ensembl: ENSG00000117751
- Entrez: 5511
- UniProt: Q12972
Shipping and storage
- Shipping: Normally shipped at ambient temperatureStorage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
References (6)
- Alternating binding and p97-mediated dissociation of SDS22 and I3 recycles active PP1 between holophosphatasesProc Natl Acad Sci U S A , 2024 Aug 29; 121(36):e2408787121. Epub 2024 Aug 292024 Aug 29
- PP1 regulatory subunit NIPP1 regulates transcription of E2F1 target genes following DNA damageCancer Sci , 2021 May 3; 112(7):2739-2752. Epub 2021 May 32021 May 3
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Researcher Contributions
Join the Explorer Program Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order. Read more...
Alternative antibodies
Corresponding antigens
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Learn how we validate our antibodies, how we secure their reproducibility, and why we apply enhanced validation. Our antibodies are validated in IHC, ICC-IF, and WB.