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HPA024705
vacuolar protein sorting 37 homolog A (S. cerevisiae)

Anti-VPS37A Antibody

Polyclonal Antibody against HUMAN VPS37A

0.4 mg/ml
In Stock (10+)
Delivery time: Ready to Ship
4 570,0 kr
Lowest price during the previous 30 days before discount: 4 352,0 kr
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Immunohistochemical staining of human cerebral cortex shows moderate to strong nuclear positivity in neurons.

Immunohistochemical staining of human cerebral cortex shows moderate to strong nuclear positivity in neurons.

Product details

  • Target protein: vacuolar protein sorting 37 homolog A (S. cerevisiae)
  • Target gene: VPS37A
  • Verified species reactivity: Human, Mouse, Rat
  • Interspecies information: Highest antigen sequence indentity to the following orthologs: MOUSE - ENSMUSG00000031600 (92%)RAT - ENSRNOG00000012001 (92%)
  • Clonality: Polyclonal
  • Isotype: IgG
  • Host: Rabbit
  • Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
    Material Safety Data Sheet
  • Purification method: Affinity purified using the PrEST antigen as affinity ligand
  • Antigen sequence: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • DVPDAFPELSELSVSQLTDMNEQEEVLLEQFLTLPQLKQIITDKDDLVKSIEELARKNLLLEPSLEAKRQTVLDKYELLTQMKSTF
  • Notes: Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Current lot

  • Unit size: 100µl
  • Current lot: A117681
  • Concentration: 0.4 mg/ml

Applications

  • Immunofluorescence in Cell Lines (ICC-IF) recommended conditions
    • Working concentration: 0.25-2 µg/ml
    • Fixation/Permeabilization: PFA/Triton X-100
  • Immunohistochemistry (IHC) recommended conditions
    • Dilution: 1:200 - 1:500
    • Retrieval method: HIER pH6
  • Western Blot (WB) recommended conditions
    • Working concentration: 0.04-0.4 µg/ml

Validation

Target information

  • Protein name: vacuolar protein sorting 37 homolog A (S. cerevisiae)
  • Gene name: VPS37A
  • Alternative gene names: FLJ32642HCRP1PQBP2SPG53
  • Ensembl: ENSG00000155975
  • Entrez: 137492
  • UniProt: Q8NEZ2

Shipping and storage

  • Shipping: Normally shipped at ambient temperatureStorage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References (2)

  • Unveiling the physiological impact of ESCRT-dependent autophagosome closure by targeting the VPS37A ubiquitin E2 variant-like domainHamamoto K, Liang X, Ito A, Lanza M, Bui V, Zhang J, Opozda DM, Hattori T, Chen L, Haddock D, Imamura F, Wang HG, Takahashi YCell Rep , 2024 Nov 27; 43(12):115016. Epub 2024 Nov 272024 Nov 27
  • Characterization data on the Human Protein AtlasThis antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.Human Protein Atlas

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Researcher Contributions

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