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Anti-BAIAP2 Antibody
Polyclonal Antibody against HUMAN BAIAP2
0.2 mg/ml
Immunohistochemical staining of human cerebellum, cerebral cortex, fallopian tube and prostate using Anti-BAIAP2 antibody HPA023310 (A) shows similar protein distribution across tissues to independent antibody HPA027421 (B).
Product details
- Target protein: BAI1-associated protein 2
- Target gene: BAIAP2
- Verified species reactivity: Human, Rat
- Interspecies information: Highest antigen sequence indentity to the following orthologs: MOUSE - ENSMUSG00000025372 (92%)RAT - ENSRNOG00000004049 (92%)
- Clonality: Polyclonal
- Isotype: IgG
- Host: Rabbit
- Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
Material Safety Data Sheet - Purification method: Affinity purified using the PrEST antigen as affinity ligand
- Antigen sequence: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
- WFPFSYTRVLDSDGSDRLHMSLQQGKSSSTGNLLDKDDLAIPPPDYGAASRAFPAQTASGFKQRPYSVAVPAFSQGLDDYGARSMSRNP
- Notes: Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Current lot
- Unit size: 100µl
- Current lot: 000023885
- Concentration: 0.2 mg/ml
Applications
- Immunofluorescence in Cell Lines (ICC-IF) recommended conditions
- Working concentration: 0.25-2 µg/ml
- Fixation/Permeabilization: PFA/Triton X-100
- Immunohistochemistry (IHC) recommended conditions
- Dilution: 1:50 - 1:200
- Retrieval method: HIER pH6
- Western Blot (WB) recommended conditions
- Working concentration: 0.04-0.4 µg/ml
Validation
- Immunofluorescence in Cell Lines (ICC-IF) All ICC-IF characterization data for HPA023310 on the Human Protein Atlas
- Immunohistochemistry (IHC)Validation of protein expression in IHC by comparing independent antibodies targeting different epitopes of the protein. Validated against independent antibody Anti-BAIAP2 HPA027421 All IHC characterization data in 44 normal tissues and 20 cancers for HPA023310 on the Human Protein Atlas
- Western Blot (WB)Validated in Western blot using relevant lysates (see Western blot application images above)
Target information
- Protein name: BAI1-associated protein 2
- Gene name: BAIAP2
- Alternative gene names: BAP2
- Ensembl: ENSG00000175866
- Entrez: 10458
- UniProt: Q9UQB8
Shipping and storage
- Shipping: Normally shipped at ambient temperatureStorage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
References (10)
- BIN1 regulates actin-membrane interactions during IRSp53-dependent filopodia formationCommun Biol , 2024 May 9; 7:549. Epub 2024 May 92024 May 9
- Tunnelling nanotube formation is driven by Eps8/IRSp53‐dependent linear actin polymerizationEMBO J , 2023 Nov 27; 42(24):e113761. Epub 2023 Nov 272023 Nov 27
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Researcher Contributions
Join the Explorer Program Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order. Read more...
Alternative antibodies
Corresponding antigens
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Learn how we validate our antibodies, how we secure their reproducibility, and why we apply enhanced validation. Our antibodies are validated in IHC, ICC-IF, and WB.