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Anti-ATXN2 Antibody
Polyclonal Antibody against HUMAN ATXN2
0.1 mg/ml
Immunohistochemistry analysis in human testis and skeletal muscle tissues using HPA021146 antibody. Corresponding ATXN2 RNA-seq data are presented for the same tissues.
Product details
- Target protein: ataxin 2
- Target gene: ATXN2
- Verified species reactivity: Human
- Interspecies information: Highest antigen sequence indentity to the following orthologs: MOUSE - ENSMUSG00000042605 (90%)RAT - ENSRNOG00000001256 (91%)
- Clonality: Polyclonal
- Isotype: IgG
- Host: Rabbit
- Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
Material Safety Data Sheet - Purification method: Affinity purified using the PrEST antigen as affinity ligand
- Antigen sequence: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
- RHPRNHRVSAGRGSISSGLEFVSHNPPSEAATPPVARTSPSGGTWSSVVSGVPRLSPKTHRPRSPRQNSIGNTPSGPVLASPQAGIIPTEAVAMPIPAASPTPASPASNRAVTPSSEAKDSRLQDQRQNSPAGNKENIKPNET
- Notes: Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Current lot
- Unit size: 100µl
- Current lot: 000055489
- Concentration: 0.1 mg/ml
Applications
- Immunohistochemistry (IHC) recommended conditions
- Dilution: 1:500 - 1:1000
- Retrieval method: HIER pH6
- Western Blot (WB) recommended conditions
- Working concentration: 0.04-0.4 µg/ml
Validation
- Immunohistochemistry (IHC)Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues. Validation of protein expression in IHC by comparing independent antibodies targeting different epitopes of the protein. Validated against independent antibodies Anti-ATXN2 HPA018295 All IHC characterization data in 44 normal tissues and 20 cancers for HPA021146 on the Human Protein Atlas
- Western Blot (WB)Validated in Western blot using relevant lysates (see Western blot application images above)
Target information
- Protein name: ataxin 2
- Gene name: ATXN2
- Alternative gene names: ATX2, SCA2, TNRC13
- Ensembl: ENSG00000204842
- Entrez: 6311
- UniProt: Q99700
Shipping and storage
- Shipping: Normally shipped at ambient temperatureStorage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
References (3)
- MTSS1/Src family kinase dysregulation underlies multiple inherited ataxiasProc Natl Acad Sci U S A , 2018 Dec 7; 115(52):E12407-E12416. Epub 2018 Dec 72018 Dec 7
- Intersection of calorie restriction and magnesium in the suppression of genome-destabilizing RNA–DNA hybridsNucleic Acids Res , 2016 Aug 29; 44(18):8870-8884. Epub 2016 Aug 292016 Aug 29
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Researcher Contributions
Join the Explorer Program Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order. Read more...
Alternative antibodies
Corresponding antigens
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Learn how we validate our antibodies, how we secure their reproducibility, and why we apply enhanced validation. Our antibodies are validated in IHC, ICC-IF, and WB.