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Anti-PNCK Antibody
Polyclonal Antibody against HUMAN PNCK
0.05 mg/ml
Immunohistochemical staining of human cerebral cortex shows moderate positivity in neurons and neuropil.
Product details
- Target protein: pregnancy up-regulated nonubiquitous CaM kinase
- Target gene: PNCK
- Verified species reactivity: Human
- Interspecies information: Highest antigen sequence indentity to the following orthologs: MOUSE - ENSMUSG00000002012 (96%)RAT - ENSRNOG00000058317 (96%)
- Clonality: Polyclonal
- Isotype: IgG
- Host: Rabbit
- Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
Material Safety Data Sheet - Purification method: Affinity purified using the PrEST antigen as affinity ligand
- Antigen sequence: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
- MLLLKKHTEDISSVYEIRERLGSGAFSEVVLAQERGSAHLVALKCIPKKALRGKEALVENEIAVLRRISHPN
- Notes: Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Current lot
- Unit size: 100µl
- Current lot: 000014379
- Concentration: 0.05 mg/ml
Applications
- Immunofluorescence in Cell Lines (ICC-IF) recommended conditions
- Working concentration: 0.25-2 µg/ml
- Fixation/Permeabilization: PFA/Triton X-100
- Immunohistochemistry (IHC) recommended conditions
- Dilution: 1:20 - 1:50
- Retrieval method: HIER pH6
Validation
- Immunofluorescence in Cell Lines (ICC-IF) All ICC-IF characterization data for HPA007458 on the Human Protein Atlas
- Immunohistochemistry (IHC) All IHC characterization data in 44 normal tissues and 20 cancers for HPA007458 on the Human Protein Atlas
Target information
- Protein name: pregnancy up-regulated nonubiquitous CaM kinase
- Gene name: PNCK
- Alternative gene names: CaMK1b, MGC45419
- Ensembl: ENSG00000130822
- Entrez: 139728
- UniProt: Q6P2M8
Shipping and storage
- Shipping: Normally shipped at ambient temperatureStorage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
References (3)
- Expression of Pregnancy Up-regulated Non-ubiquitous Calmodulin Kinase (PNCK) in Hepatocellular CarcinomaCancer Genomics Proteomics , 2020 Nov 3; 17(6):747-755. Epub 2020 Nov 32020 Nov 3
- PNCK depletion inhibits proliferation and induces apoptosis of human nasopharyngeal carcinoma cells in vitro and in vivoJ Cancer , 2019 Dec 3; 10(27):6925-6932. Epub 2019 Dec 32019 Dec 3
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Researcher Contributions
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Corresponding antigens
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