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Anti-SCGN Antibody
Polyclonal Antibody against HUMAN SCGN
0.2 mg/ml
Immunohistochemistry analysis in human pancreas and fallopian tube tissues using HPA006641 antibody. Corresponding SCGN RNA-seq data are presented for the same tissues.
Product details
- Target protein: secretagogin, EF-hand calcium binding protein
- Target gene: SCGN
- Verified species reactivity: Human, Mouse
- Interspecies information: Highest antigen sequence indentity to the following orthologs: MOUSE - ENSMUSG00000021337 (96%)RAT - ENSRNOG00000016628 (96%)
- Clonality: Polyclonal
- Isotype: IgG
- Host: Rabbit
- Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
Material Safety Data Sheet - Purification method: Affinity purified using the PrEST antigen as affinity ligand
- Antigen sequence: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
- RDLFLHHKKAISEAKLEEYTGTMMKIFDRNKDGRLDLNDLARILALQENFLLQFKMDACSTEERKRDFEKIFAYYDVSKTGALEGPEVDGFVKDMMELVQPSISGVDLDKFREILLRHCDVNKDGKIQKSELALCLGLK
- Notes: Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Current lot
- Unit size: 100µl
- Current lot: 000041605
- Concentration: 0.2 mg/ml
Applications
- Immunohistochemistry (IHC) recommended conditions
- Dilution: 1:500 - 1:1000
- Retrieval method: HIER pH6
- Western Blot (WB) recommended conditions
- Working concentration: 0.04-0.4 µg/ml
Validation
- Immunohistochemistry (IHC)Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues. All IHC characterization data in 44 normal tissues and 20 cancers for HPA006641 on the Human Protein Atlas
- Western Blot (WB)Validated in Western blot using relevant lysates (see Western blot application images above)
Target information
- Protein name: secretagogin, EF-hand calcium binding protein
- Gene name: SCGN
- Alternative gene names: CALBL, DJ501N12.8, SECRET, SEGN
- Ensembl: ENSG00000079689
- Entrez: 10590
- UniProt: O76038
Shipping and storage
- Shipping: Normally shipped at ambient temperatureStorage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
References (18)
- Meningioma classification by immunohistochemistry: A replicability studyBrain Spine , 2022 Dec 27; 3:101711. Epub 2022 Dec 272022 Dec 27
- RNA-Seq Analysis Reveals an Essential Role of the Tyrosine Metabolic Pathway and Inflammation in Myopia-Induced Retinal Degeneration in Guinea PigsInt J Mol Sci , 2021 Nov 22; 22(22):12598. Epub 2021 Nov 222021 Nov 22
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Researcher Contributions
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Alternative antibodies
Corresponding antigens
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Learn how we validate our antibodies, how we secure their reproducibility, and why we apply enhanced validation. Our antibodies are validated in IHC, ICC-IF, and WB.