G6PD Antibody (HPA000834)

G6PD Antibody (HPA000834)

Anti-G6PD

glucose-6-phosphate dehydrogenase
Anti-G6PD
HPA000834
100 µl
$429.00
Available in stock
Clonality
Polyclonal
Alternative names
G6PD1
Species reactivity
Human
Alternative Antibodies
Close
  • Immunohistochemical staining of human testis shows strong cytoplasmic and nuclear positivity in cells in seminiferus ducts.
Immunohistochemistry
Recommended conditions:
  • Dilution: 1:2500 - 1:5000
  • Retrieval method: HIER pH6
Additional IHC Characterization Data
Access additional IHC-data for Anti-G6PD on the Human Protein Atlas. View manually annotated IHC-data from 576 human tissue samples of normal and cancer origin as well as duplicate samples from 59 clinical cell samples and cell lines.
Close
  • Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 18
    Lane 2: Human cell line RT-4
    Lane 3: Human cell line U-251MG sp
    Lane 4: Human cell line A-431
    Lane 5: Human liver tissue
    Lane 6: Human tonsil tissue
Western Blot
Recommended conditions:
  • Dilution: 1:100 - 1:250
Additional WB Characterization Data
Access the Western Blot validation data for Anti-G6PD on the Human Protein Atlas. Use the Antigen View to display the antigen location on the target protein for all splice variants.
Close
  • Immunofluorescent staining of human cell line U-2 OS shows positivity in cytoplasm.
Immunofluorescence
Recommended conditions:
  • Fixation/Permeabilization: PFA/Triton X-100
  • Working concentration: 1-4 µg/ml
Additional IF Characterization Data
Access additional IF characterization data for Anti-G6PD on the Human Protein Atlas. View manually annotated subcellular localization data in three cell lines selected based on RNA sequencing data.
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Antigen Sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence:
FQGDAFHQSDTHIFIIMGASGDLAKKKIYPTIWWLFRDGLLPENTFIVGYARSRLTVADIRKQSEPFFKATPEEKLKLEDFFARNSYVAGQYDDAASYQRLNSHMNALH
Purification Method
Affinity purified using the PrEST antigen as affinity ligand
Specificity
Specific reactivity against target PrEST antigen validated on protein array with 384 randomly selected antigens.
Interspecies Information Highest antigen sequence identity to the following orthologos:
Form
The antibodies are delivered in 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Vial Size 100 µl
Current Lot
A06361
Concentration
0.2 mg/ml
Storage Store at +4°C for short term storage (1-2 days). Long time storage is recommended at -20°C. If stored at lower temperature, aliquot to avoid repeated freezing and thawing. Working dilution samples should be discarded if not used within 12 hours.
Shipping
Normally shipped at ambient temperature
Notes
The antibody solution should be gently mixed before use. Optimal concentrations and conditions for each application should be determined by the user.

Human Protein Atlas

This antibody has been used for staining of 48 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

Kato BS, Nicholson G, Neiman M, Rantalainen M, Holmes CC, Barrett A, Uhlén M, Nilsson P, Spector TD, Schwenk JM Variance decomposition of protein profiles from antibody arrays using a longitudinal twin model. Proteome Sci 2011 Nov 17;9:73 PubMed ID: 22093360 DOI: 10.1186/1477-5956-9-73
Du W, Jiang P, Mancuso A, Stonestrom A, Brewer MD, Minn AJ, Mak TW, Wu M, Yang X TAp73 enhances the pentose phosphate pathway and supports cell proliferation. Nat Cell Biol 2013 Aug;15(8):991-1000 PubMed ID: 23811687 DOI: 10.1038/ncb2789

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If you have questions or comments regarding this antibody please contact our Scientific Support: contact@atlasantibodies.com.

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