ATP2B3 Antibody (HPA001583)

ATP2B3 Antibody (HPA001583)

Anti-ATP2B3

ATPase, Ca++ transporting, plasma membrane 3
Anti-ATP2B3
HPA001583
100 µl
$429.00
Available in stock
Clonality
Polyclonal
Alternative names
PMCA3
Species reactivity
Human
Close
  • Immunohistochemical staining of human salivary gland shows moderate membranous positivity in glandular cells.
Immunohistochemistry
Recommended conditions:
  • Dilution: 1:200 - 1:500
  • Retrieval method: HIER pH6
Additional IHC Characterization Data
Access additional IHC-data for Anti-ATP2B3 on the Human Protein Atlas. View manually annotated IHC-data from 576 human tissue samples of normal and cancer origin as well as duplicate samples from 59 clinical cell samples and cell lines.
Close
  • Immunofluorescent staining of human cell line A-431 shows positivity in plasma membrane & golgi apparatus.
Immunofluorescence
Recommended conditions:
  • Fixation/Permeabilization: PFA/Triton X-100
  • Working concentration: 1-4 µg/ml
Additional IF Characterization Data
Access additional IF characterization data for Anti-ATP2B3 on the Human Protein Atlas. View manually annotated subcellular localization data in three cell lines selected based on RNA sequencing data.
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Antigen Sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence:
IRVVKAFRSSLYEGLEKPESKTSIHNFMATPEFLINDYTHNIPLIDDTDVDENEERLRAPPPPSPNQNNNAIDSGIYLTTHVTKSATSSVFSSSPGSPLHSVETS
Purification Method
Affinity purified using the PrEST antigen as affinity ligand
Specificity
Specific reactivity against target PrEST antigen validated on protein array with 384 randomly selected antigens.
Interspecies Information Highest antigen sequence identity to the following orthologos:
Form
The antibodies are delivered in 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Vial Size 100 µl
Current Lot
R00465
Concentration
0.1 mg/ml
Storage Store at +4°C for short term storage (1-2 days). Long time storage is recommended at -20°C. If stored at lower temperature, aliquot to avoid repeated freezing and thawing. Working dilution samples should be discarded if not used within 12 hours.
Shipping
Normally shipped at ambient temperature
Notes
The antibody solution should be gently mixed before use. Optimal concentrations and conditions for each application should be determined by the user.

Human Protein Atlas

This antibody has been used for staining of 48 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

Beuschlein F, Boulkroun S, Osswald A, Wieland T, Nielsen HN, Lichtenauer UD, Penton D, Schack VR, Amar L, Fischer E, Walther A, Tauber P, Schwarzmayr T, Diener S, Graf E, Allolio B, Samson-Couterie B, Benecke A, Quinkler M, Fallo F, Plouin PF, Mantero F, Meitinger T, Mulatero P, Jeunemaitre X, Warth R, Vilsen B, Zennaro MC, Strom TM, Reincke M Somatic mutations in ATP1A1 and ATP2B3 lead to aldosterone-producing adenomas and secondary hypertension. Nat Genet 2013 Feb 17; PubMed ID: 23416519 DOI: 10.1038/ng.2550

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If you have questions or comments regarding this antibody please contact our Scientific Support: contact@atlasantibodies.com.

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