APOOL Antibody (HPA000612)

APOOL Antibody (HPA000612)

Anti-APOOL

apolipoprotein O-like
Anti-APOOL
HPA000612
100 µl
$429.00
Available in stock
Clonality
Polyclonal
Alternative names
AAIR8193, CXorf33, FAM121A, UNQ8193
Species reactivity
Human, Mouse, Rat
Close
  • Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in glandular cells.
Immunohistochemistry
Recommended conditions:
  • Dilution: 1:500 - 1:1000
  • Retrieval method: HIER pH6
Additional IHC Characterization Data
Access additional IHC-data for Anti-APOOL on the Human Protein Atlas. View manually annotated IHC-data from 576 human tissue samples of normal and cancer origin as well as duplicate samples from 59 clinical cell samples and cell lines.
Close
  • Lane 1: Marker [kDa] 207, 110, 79, 49, 32, 25, 17
    Lane 2: Human cell line RT-4
    Lane 3: Human cell line EFO-21
    Lane 4: Human cell line A-431
  • Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
    Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
Western Blot in Human Material
Recommended conditions:
  • Dilution: 1:250 - 1:500
Additional WB Characterization Data
Access the Western Blot validation data for Anti-APOOL on the Human Protein Atlas. Use the Antigen View to display the antigen location on the target protein for all splice variants.
Western Blot in Rodent Material
Recommended conditions:
  • Dilution: 1:100 - 1:500
Close
  • Immunofluorescent staining of human cell line A-431 shows positivity in mitochondria.
Immunofluorescence
Recommended conditions:
  • Fixation/Permeabilization: PFA/Triton X-100
  • Working concentration: 1-4 µg/ml
Additional IF Characterization Data
Access additional IF characterization data for Anti-APOOL on the Human Protein Atlas. View manually annotated subcellular localization data in three cell lines selected based on RNA sequencing data.
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Antigen Sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence:
ATLGATVCYPVQSVIIAKVTAKKVYATSQQIFGAVKSLWTKSSKEESLPKPKEKTKLGSSSEIEVPAKTTHVLKHSVPLPTELSSEAKTKSESTSGATQFMPDPKLMDHGQSHPED
Purification Method
Affinity purified using the PrEST antigen as affinity ligand
Specificity
Specific reactivity against target PrEST antigen validated on protein array with 384 randomly selected antigens.
Interspecies Information Highest antigen sequence identity to the following orthologos:
Form
The antibodies are delivered in 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Vial Size 100 µl
Current Lot
R00191
Concentration
0.3 mg/ml
Storage Store at +4°C for short term storage (1-2 days). Long time storage is recommended at -20°C. If stored at lower temperature, aliquot to avoid repeated freezing and thawing. Working dilution samples should be discarded if not used within 12 hours.
Shipping
Normally shipped at ambient temperature
Notes
The antibody solution should be gently mixed before use. Optimal concentrations and conditions for each application should be determined by the user.

Human Protein Atlas

This antibody has been used for staining of 48 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

Mulder J, Wernérus H, Shi TJ, Pontén F, Hober S, Uhlén M, Hökfelt T Systematically generated antibodies against human gene products: high throughput screening on sections from the rat nervous system. Neuroscience 2007 Jun 8;146(4):1689-703 PubMed ID: 17478047 DOI: 10.1016/j.neuroscience.2007.02.054
Mulder J, Björling E, Jonasson K, Wernérus H, Hober S, Hökfelt T, Uhlén M Tissue profiling of the mammalian central nervous system using human antibody-based proteomics. Mol Cell Proteomics 2009 Jul;8(7):1612-22 PubMed ID: 19351664 DOI: 10.1074/mcp.M800539-MCP200
Weber TA, Koob S, Heide H, Wittig I, Head B, van der Bliek A, Brandt U, Mittelbronn M, Reichert AS APOOL Is a Cardiolipin-Binding Constituent of the Mitofilin/MINOS Protein Complex Determining Cristae Morphology in Mammalian Mitochondria PLoS One 8(5):e63683. Epub 2013/05/21. PubMed ID: 23704930 DOI: 10.1371/journal.pone.0063683

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100 researchers are working within the Human Protein Atlas project. Our Scientific Support Team provides you with answers or guide you to the right people

If you have questions or comments regarding this antibody please contact our Scientific Support: contact@atlasantibodies.com.

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