ADSL Antibody (HPA000525)

ADSL Antibody (HPA000525)

Anti-ADSL

adenylosuccinate lyase
Anti-ADSL
HPA000525
100 µl
$429.00
Available in stock
Clonality
Polyclonal
Alternative names
Species reactivity
Human
Close
  • Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in reaction center cells.
Immunohistochemistry
Recommended conditions:
  • Dilution: 1:50 - 1:200
  • Retrieval method: HIER pH6
Additional IHC Characterization Data
Access additional IHC-data for Anti-ADSL on the Human Protein Atlas. View manually annotated IHC-data from 576 human tissue samples of normal and cancer origin as well as duplicate samples from 59 clinical cell samples and cell lines.
Close
  • Lane 1: Marker [kDa] 207, 110, 79, 49, 32, 25, 17
    Lane 2: Human cell line RT-4
    Lane 3: Human cell line EFO-21
    Lane 4: Human cell line A-431
    Lane 5: Human liver tissue
    Lane 6: Human tonsil tissue
Western Blot
Recommended conditions:
  • Dilution: 1:250 - 1:500
Additional WB Characterization Data
Access the Western Blot validation data for Anti-ADSL on the Human Protein Atlas. Use the Antigen View to display the antigen location on the target protein for all splice variants.
Close
  • Immunofluorescent staining of human cell line U-251MG shows positivity in cytoplasm.
Immunofluorescence
Recommended conditions:
  • Fixation/Permeabilization: PFA/Triton X-100
  • Working concentration: 1-4 µg/ml
Additional IF Characterization Data
Access additional IF characterization data for Anti-ADSL on the Human Protein Atlas. View manually annotated subcellular localization data in three cell lines selected based on RNA sequencing data.
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Antigen Sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence:
RRICLAEAFLTADTILNTLQNISEGLVVYPKVIERRIRQELPFMATENIIMAMVKAGGSRQDCHEKIRVLSQQAASVVKQEGGDNDLIERIQVDAYFSPIHSQLDHLLDPSSFTGRASQQVQRFLEEEVYPLLKPYESVMKVKA
Purification Method
Affinity purified using the PrEST antigen as affinity ligand
Specificity
Specific reactivity against target PrEST antigen validated on protein array with 384 randomly selected antigens.
Interspecies Information Highest antigen sequence identity to the following orthologos:
Form
The antibodies are delivered in 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Vial Size 100 µl
Current Lot
A08758
Concentration
0.1 mg/ml
Storage Store at +4°C for short term storage (1-2 days). Long time storage is recommended at -20°C. If stored at lower temperature, aliquot to avoid repeated freezing and thawing. Working dilution samples should be discarded if not used within 12 hours.
Shipping
Normally shipped at ambient temperature
Notes
The antibody solution should be gently mixed before use. Optimal concentrations and conditions for each application should be determined by the user.

Human Protein Atlas

This antibody has been used for staining of 48 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

Baresova V, Skopova V, Sikora J, Patterson D, Sovova J, Zikanova M, Kmoch S Mutations of ATIC and ADSL affect purinosome assembly in cultured skin fibroblasts from patients with AICA-ribosiduria and ADSL deficiency. Hum Mol Genet 2012 Apr 1;21(7):1534-43 PubMed ID: 22180458 DOI: 10.1093/hmg/ddr591
Stadler C, Hjelmare M, Neumann B, Jonasson K, Pepperkok R, Uhlén M, Lundberg E Systematic validation of antibody binding and protein subcellular localization using siRNA and confocal microscopy. J Proteomics 2012 Apr 3;75(7):2236-51 PubMed ID: 22361696 DOI: 10.1016/j.jprot.2012.01.030

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If you have questions or comments regarding this antibody please contact our Scientific Support: contact@atlasantibodies.com.

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