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Analysis of brain organoid using our anti-HOPX antibody

Luongo R, et al. Protocol for generating human cortical organoids enriched in outer radial glia by guided differentiation. STAR Protoc. 2024 Sep 20;5(3):103280. PMID: 39213150.

The generation of human pluripotent stem cell (hPSC)-derived brain organoids is continuously refined, enhancing their reproducibility and complexity.

This newly published study presents a guided differentiation protocol for generating cortical forebrain organoids and cortico-pericyte (CP) assembloids composed of a robust outer radial glia (oRG) population and an expanded outer subventricular zone (oSVZ).

It describes the steps to generate hPSC-derived cortical organoids (COs), cortical pericytes, and CP assembloids. Moreover, we outline the procedures to characterize the organoids by immunostaining and to perform single-cell dissociation.

The aim is to mimic human brain development more accurately, as oRG cells are critical for cortical expansion and gyrification.

This study featured immunocytochemistry (ICC) and immunofluorescence (IF) techniques on brain organoids using Atlas Antibodies' anti-HOPX antibody (Cat. HPA030180).

immunocytochemistry (ICC) and immunofluorescence (IF) techniques on brain organoids using Atlas Antibodies' anti-HOPX antibody (Cat. HPA030180).

 

The results indicate that this method leads to organoids enriched in oRG cells, offering a valuable model to study neurodevelopmental processes and brain evolution, and potentially improve research on conditions linked to cortical malformations.

Read the full study

  

Anti-HOPX Antibody
Anti-HOPX Antibody

Anti-HOPX Antibody

HPA030180-100UL
In Stock (10+)
4 311,0 kr
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