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AtlasPlex
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AtlasPlex: Custom Multiplex Immunohistochemistry (IHC) Kit: Unlimited Targets. Less Complexity. Faster Discoveries.

Simplified Multiplex IHC

AtlasPlex is a flexible, ready-to-use multiplexing immunohistochemistry assay that brings high-throughput, iterative mIHC to FFPE tissue with unmatched clarity.

Designed to detect up to five biomarkers per day, AtlasPlex combines a complete, pre-validated TSA kit with 3 or 5 pre-biotinylated antibodies and tyramide dyes, eliminating the need for secondary antibodies and complex optimization. 

Choose from an unparalleled selection of more than 12,000 Human Protein Atlas–validated targets, you can design fully customizable panels without pre-set restrictions, ensuring maximum flexibility across diverse research areas. By expanding marker capacity, streamlining workflows, and delivering robust, reproducible signals, AtlasPlex allows you to focus on discovery rather than technical hurdles.

Get all the technical specification. 

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Order Your Custom Kit Today →

 

Visualize More with Flexible Multiplex IHC Panels

Achieve brilliant, reproducible results with the Human Protein Atlas' catalog flexibility. 

 

 

1) Build your own kit choosing from over 12,000 targets

With AtlasPlex, you have the flexibility to build your own custom multiplex IHC kit by selecting from over 12,000 validated targets. All antibodies are rigorously IHC-validated and supported by comprehensive data, including extensive validation images of tissue and organs from the Human Protein Atlas, ensuring high confidence in target detection and reliable, reproducible results for your research.

 

🔹 Choose among ~12,000 targets

🔹 No primary antibody host species restrictions

🔹 Up to 5-plex/day for fast, flexible experimental design

🔹 Bright, permanent tyramide signal, no fading overtime

🔹 Low background, high specificity

🔹 Fully compatible with standard fixed (FFPE) tissue workflows

🔹Ideal for spatial biology, tumor microenvironment, and biomarker discovery

 

2) Workflow: from sample to results in one day

 

AtlasPlex workflow

AtlasPlex enables iterative multiplexed immunohistochemistry (mIHC) for 3-5 proteins detection in FFPE tissue.

  1. Smart Complexing: Biotinylated primary antibodies (Ab1) are pre-bound to HRP, setting the stage for powerful signal amplification.
  2. Targeted Tissue Binding: Apply the Ab1-HRP complex directly to fixed tissue. Binding is precise, reliable, and reproducible. 
  3. Amplification: Tyramide-based signal amplification deposits a bright, permanent fluorescent signal at each target site, ensuring crisp, high-contrast images. 
  4. Enzyme Quenching: Deactivate HRP quickly and cleanly. This rapid, gentle step completely replaces the need for harsh, tissue-damaging antibody stripping, ensuring no interference in downstream rounds. 
  5. Repeat: Run multiple antibody cycles (Ab2...Ab5) with only 1.5 hours per cycle. Up to 5-plex per day. 
  6. Brilliant Imaging: Capture your results using standard fluorescence microscopy. Reveal distinct biomarker landscapes with unmatched clarity.

Watch latest AtlasPlex webinar

 

3) Clear spectral coverage for enhanced visualization

Multichannel Fluorescent Imaging

Using Standard Wide-Field Microscope

AtlasPlex (3 Plex) included in the Kit

Tyramide 488
Tyramide 555
Tyramide 647
 

AtlasPlex (5 Plex) included in the Kit

Tyramide 488
Tyramide 555
Tyramide 647
Tyramide CF430
Tyramide 594

 

4) Less Time. Enhanced Sensitivity. Unlock Flexibility with AtlasPlex 

Simplify Setup & Save Weeks

Stop assembling complex protocols from scratch. AtlasPlex delivers everything you need in one box, with pre-biotinylated antibodies and optimized TSA reagents, dramatically reducing setup and optimization time.

Superior Sensitivity for Low-Abundance Proteins

Detect even challenging, low-expression targets. AtlasPlex utilizes powerful TSA amplification with an integrated quencher to maximize signal-to-noise ratio.

Unprecedented HPA Flexibility 

Don't be limited by pre-set panels. AtlasPlex offers unparalleled flexibility, allowing you to incorporate a wide array of IHC-validated antibodies from the Human Protein Atlas, covering most human genes.

Results in a Day Confident 5-Plex Staining

Maximize insights from your precious paraffin-embedded tissue sections. AtlasPlex is optimized for robust multiplexing, enabling clear visualization of up to 5 targets simultaneously in a single day.

Ready to Transform Your Multiplex Tissue Imaging?

🎯 AtlasPlex: Your Complete Solution for Brilliant, Reproducible Multiplex Tissue Staining

 

Build Your Kit →

 

AtlasPlex Technical Questions Answered

 

  • Is AtlasPlex beginner-friendly?

    Yes, AtlasPlex is user-friendly and suitable for beginners, with ready-to-use kits that reduce the need for extensive protocol optimization.​